De Villota was found dead in a hotel early on Friday morning in Seville.
De Villota was returning to the pits at Duxford Aerodrome in July 2012 when her Marussia car struck a team support truck. She sustained serious skull injuries and lost the use of her right eye.
‘Maria left us while she was sleeping, approximately at 6am, as a consequence of the neurological injuries that she suffered in July of 2012, according to what the forensic doctor has told us,’ read a statement released by De Villota’s family.
‘Maria is gone, but she has left us a very clear message of joy and hope, which is helping the family move on in these moments.’
The accident remains under investigation by the British Health and Safety Executive.
Formula 1 will pay tribute to De Villota at the Japanese Grand Prix on Sunday.
A statement released by the Grand Prix Drivers’ Association said that a minute’s silence will be held prior to the drivers’ parade, while the podium will also be dedicated to De Villota.